Tetrazine-PEG Overview
Tetrazine-PEG combines a
tetrazine group with a
PEG chain for efficient
bioorthogonal click chemistry. The
Tetrazine ligation involves a fast
inverse electron demand Diels-Alder (IEDDA) reaction with
trans-cyclooctene (TCO) or other strained alkenes. This reaction occurs at a second-order rate of 2000 M−1-s−1, quickly modifying biomolecules and eliminating nitrogen gas. It works even at low concentrations and performs well in aqueous media, making it perfect for live-cell labeling and bio-entity modification.
Key Features:
- Fast Tetrazine Ligation: Quickly reacts with TCO or norbornenes, ensuring rapid modifications.
- PEG Chain: Enhances solubility and biocompatibility, reducing immune responses.
- Quick Reactions: Works efficiently in aqueous media, ideal for real-time biomolecule labeling.
Applications:
- Bioconjugation: Modifies proteins and peptides at low concentrations with high precision.
- Drug Delivery: Improves drug solubility and delivery efficiency.
- Live-Cell Labeling: Perfect for fast and accurate labeling in live-cell studies.
- Imaging: Enables precise tracking of biomolecules in diagnostics and research.
Advantages:
- High Specificity: Reacts only with dienophiles like TCO, ensuring precise targeting.
- Speed: Rapid reaction rates make it ideal for time-sensitive applications.
- Better Biocompatibility: PEG enhances solubility and reduces unwanted immune reactions.
Tetrazine-PEG is ideal for researchers needing fast, reliable solutions for
bioconjugation,
drug delivery, and
live-cell imaging.
Ref:
- Segawa, S., Wu, J., Kwok, R.T., Wong, T.T., He, X. and Tang, B.Z., 2024. Co‐aggregation as A Simple Strategy for Preparing Fluorogenic Tetrazine Probes with On‐Demand Fluorogen Selection. Angewandte Chemie International Edition, 63(11), p.e202313930.
