Bis-PEG-NHS or Bis-NHS-PEG is a cross-linker with a PEG chain and two NHS ester groups. It binds to primary amines for bioconjugation, labeling, and surface modification, enhancing stability and solubility.
Bis-PEG-NHS
Bis-NHS-PEG, or Bis-PEG-NHS, serves as a cross-linking reagent. It has a PEG chain with two NHS ester groups. The length of the PEG can vary, changing its flexibility and solubility.
The NHS ester groups bind directly to primary amines. This forms stable amide bonds with proteins, peptides, or other molecules.
Researchers often choose Bis-NHS-PEG for labeling, bioconjugation, and cross-linking. It also works well for surface modification. The PEG structure adds flexibility and cuts down on unwanted interactions. As a result, it boosts the stability and solubility of the conjugates.