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APDye Fluor 555 (AF555, Alexa Fluor 555) is a bright, water-soluble orange fluorescent dye. It works with 532 nm or 555 nm laser lines and TRITC filter sets. With peak excitation/emission at 555/565 nm, it enables bright, stable conjugates for sensitive detection. Ideal for flow cytometry, fluorescence microscopy, and ELISA, it offers higher fluorescence and better photostability than Cy3 alternatives.

APDye Fluor 555

Cat# Name Structure M.W. Purity Pricing
AP12433APDye 555 Alkyne
Quick viewAPDye 555 Alkyne
884.06≥95% Pricing
AP12639CalFluor 555 Azide
Quick viewCalFluor 555 Azide
891.09≥95% Pricing
AP12431APDye 555 Azide
Quick viewAPDye 555 Azide
928.25 (protonated)≥95% Pricing
AP12429APDye 555 NHS ester
Quick viewAPDye 555 NHS ester
944.07 (protonated)≥95% Pricing
AP14425AP555 carboxylic acid
Quick viewAP555 carboxylic acid
961.29≥95% Pricing
AP14038APDye 555 Tyramide
Quick viewAPDye 555 Tyramide
966.16 (protonated)≥95% Pricing
AP12430APDye 555 Maleimide
Quick viewAPDye 555 Maleimide
969.12≥95% Pricing
AP12432APDye 555 Picolyl Azide
Quick viewAPDye 555 Picolyl Azide
1049.21≥95% Pricing
AP12667APDye 555 Azide Plus
Quick viewAPDye 555 Azide Plus
1067.28 (protonated)≥95% Pricing
AP12434APDye 555 DBCO
Quick viewAPDye 555 DBCO
1091.29≥95% Pricing

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    APDye Fluor 555 (AF555, Alexa Fluor 555) Overview

    Key Features:

    • Bright Orange Fluorescent Dye: Water-soluble and highly visible.
    • Laser Compatibility: Optimized for 532 nm and 555 nm lasers, with TRITC filter sets. Peak excitation/emission at 555/565 nm.
    • pH Stability: Performs reliably across pH 4-10.
    • High Molar Ratio Conjugation: Attaches to proteins without self-quenching, delivering brighter results and sensitive detection.

    Performance Advantage:

    • Superior to Cy3 Alternatives: Matches Cy3 spectrum, compatible with Cy3 filters.
    • Higher Fluorescence & Photostability: Longer image capture times.

    Applications:

    • Versatile Use: Ideal for flow cytometry, fluorescence microscopy, immunohistochemistry, ELISA, indirect FISH, and more.

    Explore more about Cy3b NHS ester, Cy3 azide, and Cy3 NHS ester.

    Why Choose APDye Fluor 555? Brighter, longer-lasting, and versatile — perfect for a wide range of scientific applications.

    Ref:

    Berlier JE, Rothe A, Buller G, et al. Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates. Journal of Histochemistry & Cytochemistry. 2003;51(12):1699-1712.