bioanalytical services

Bioanalysis Services: Supporting Your Drug Discovery/Development With Advanced Technologies and Dedicated Experts

AxisPharm delivers a full range of bioanalytical services to support drug discovery, clinical research, and biomarker analysis. We focus on the characterization, measurement, and analysis of biological molecules and compounds essential to life sciences.

Located in San Diego, AxisPharm has over 25 years of expertise in bioanalytical LC/MS/MS services. Our skilled team specializes in developing and validating methods for PK/TK analysis of small molecules, proteins, peptides, and metabolites. We use advanced techniques like LC-MS/MS (HPLC, UPLC, on-line SPE), HPLC/UV, and HPLC/FL. We provide expert bioanalytical support from early discovery to late-stage development, ensuring precise results every step of the way.

Instruments and Technologies Used

1. Mass Spectrometry (MS) Services

AxisPharm provides services that focus on the analysis of biomolecules using mass spectrometry techniques, such as liquid chromatography-mass spectrometry (LC-MS) or gas chromatography-mass spectrometry (GC-MS). Our MS services cover metabolomics, proteomics, small molecule analysis, drug quantification, and structural characterization.

2. Chromatography Services

Chromatography-based services involve the separation and analysis of complex mixtures of biomolecules. Techniques include high-performance liquid chromatography (HPLC), gas chromatography (GC), and ion chromatography (IC). These services are commonly used for separation, purification, and quantification of small molecules, proteins, peptides, and other compounds.

3. Nucleic Acid Analysis Services

These services focus on the analysis of nucleic acids using techniques such as polymerase chain reaction (PCR), DNA sequencing, gene expression analysis, genotyping, and nucleic acid isolation and purification. These services are essential for applications such as genetic testing, mutation analysis, and gene expression profiling.

4. Microscopy and Imaging Services

Microscopy and imaging services involve the use of microscopy techniques for visualization and analysis of biological samples. This includes fluorescence microscopy, confocal microscopy, electron microscopy, and digital imaging. These services are employed for cellular imaging, subcellular localization, histology, and other imaging-based analyses.

5.Flow Cytometry

Flow cytometry is a technique that analyzes individual cells or particles as they pass through a laser-based system. It allows for the characterization, quantification, and sorting of cells based on their size, morphology, and specific markers. Flow cytometry is commonly used in immunology, cell biology, and clinical diagnostics.

6. Next-Generation Sequencing (NGS) Services

NGS services involve high-throughput DNA or RNA sequencing using technologies like Illumina, Ion Torrent, or PacBio sequencing platforms. NGS services are essential for genome sequencing, transcriptomics, epigenomics, and metagenomics.

7. Enzyme-Linked Immunosorbent Assay (ELISA)

ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used technique in bioanalysis for the detection and quantification of specific proteins, peptides, antibodies, hormones, cytokines, and other analytes in biological samples. It utilizes the specific binding of antibodies to target analytes. ELISA is a versatile and sensitive technique used in various fields, including clinical diagnostics, pharmaceutical research, immunology, and molecular biology. It enables the detection and quantification of specific analytes in biological samples, aiding in disease diagnosis, biomarker discovery, therapeutic monitoring, and research investigations.

8. Polymerase Chain Reaction (PCR)

PCR is a widely used molecular biology technique that allows for the amplification of specific DNA or RNA sequences. It is utilized for applications such as gene expression analysis, genotyping, mutation detection, and infectious disease diagnosis.

9.Other Spectroscopy Services

Spectroscopy-based services utilize techniques that measure the interaction of biomolecules with light, including UV-visible spectroscopy, infrared spectroscopy (IR), nuclear magnetic resonance spectroscopy (NMR), and fluorescence spectroscopy. These services are employed for structural analysis, chemical characterization, and biomolecular interactions.

We also provide pre-formulation and formulation stability, degradation monitoring, and so on.

Our bioanalytical laboratory can perform all the sample treatment procedures including protein precipitation, solid phase extraction, and liquid-liquid extraction.

To submit PK samples, we will need the following items included in the package:

  • At least 1 mg pure compound
  • About 1 ml formulated solution that is the same as used for animal administration (esp. for none PO delivery)
  • At least 2 ml blank serum or biological fluid the same type as the samples
  • At least 100 µl sample amount for each sample

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